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anti stc2  (Proteintech)


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    Structured Review

    Proteintech anti stc2
    Anti Stc2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+stc2+antibody/Stanniocalcin+2+Antibody/pm41408929-93-21-23
    Average 93 stars, based on 26 article reviews
    anti stc2 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Modification:

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial–Mesenchymal Transition
    Article Snippet: .. Dulbecco's Modified Eagle's Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham's F12 (Cat. 11320033), Leibovitz's L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti- β -Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) .. The clinical tumor and corresponding normal specimens were collected from 98 cases of pancreatic cancer patients, who were operated at the Fuzhou General Hospital (Dongfang Hospital) of Xiamen University.

    RNA Extraction:

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial–Mesenchymal Transition
    Article Snippet: .. Dulbecco's Modified Eagle's Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham's F12 (Cat. 11320033), Leibovitz's L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti- β -Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) .. The clinical tumor and corresponding normal specimens were collected from 98 cases of pancreatic cancer patients, who were operated at the Fuzhou General Hospital (Dongfang Hospital) of Xiamen University.

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial-Mesenchymal Transition.
    Article Snippet: .. Dulbecco’sModified Eagle’s Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham’s F12 (Cat. 11320033), Leibovitz’s L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti-β-Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) 2.2. ..

    cDNA Synthesis:

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial–Mesenchymal Transition
    Article Snippet: .. Dulbecco's Modified Eagle's Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham's F12 (Cat. 11320033), Leibovitz's L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti- β -Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) .. The clinical tumor and corresponding normal specimens were collected from 98 cases of pancreatic cancer patients, who were operated at the Fuzhou General Hospital (Dongfang Hospital) of Xiamen University.

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial-Mesenchymal Transition.
    Article Snippet: .. Dulbecco’sModified Eagle’s Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham’s F12 (Cat. 11320033), Leibovitz’s L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti-β-Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) 2.2. ..

    Real-time Polymerase Chain Reaction:

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial–Mesenchymal Transition
    Article Snippet: .. Dulbecco's Modified Eagle's Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham's F12 (Cat. 11320033), Leibovitz's L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti- β -Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) .. The clinical tumor and corresponding normal specimens were collected from 98 cases of pancreatic cancer patients, who were operated at the Fuzhou General Hospital (Dongfang Hospital) of Xiamen University.

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial-Mesenchymal Transition.
    Article Snippet: .. Dulbecco’sModified Eagle’s Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham’s F12 (Cat. 11320033), Leibovitz’s L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti-β-Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) 2.2. ..

    SYBR Green Assay:

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial–Mesenchymal Transition
    Article Snippet: .. Dulbecco's Modified Eagle's Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham's F12 (Cat. 11320033), Leibovitz's L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti- β -Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) .. The clinical tumor and corresponding normal specimens were collected from 98 cases of pancreatic cancer patients, who were operated at the Fuzhou General Hospital (Dongfang Hospital) of Xiamen University.

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial-Mesenchymal Transition.
    Article Snippet: .. Dulbecco’sModified Eagle’s Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham’s F12 (Cat. 11320033), Leibovitz’s L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti-β-Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) 2.2. ..

    Concentration Assay:

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial–Mesenchymal Transition
    Article Snippet: .. Dulbecco's Modified Eagle's Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham's F12 (Cat. 11320033), Leibovitz's L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti- β -Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) .. The clinical tumor and corresponding normal specimens were collected from 98 cases of pancreatic cancer patients, who were operated at the Fuzhou General Hospital (Dongfang Hospital) of Xiamen University.

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial-Mesenchymal Transition.
    Article Snippet: .. Dulbecco’sModified Eagle’s Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham’s F12 (Cat. 11320033), Leibovitz’s L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti-β-Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) 2.2. ..

    Cell Cycle Assay:

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial–Mesenchymal Transition
    Article Snippet: .. Dulbecco's Modified Eagle's Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham's F12 (Cat. 11320033), Leibovitz's L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti- β -Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) .. The clinical tumor and corresponding normal specimens were collected from 98 cases of pancreatic cancer patients, who were operated at the Fuzhou General Hospital (Dongfang Hospital) of Xiamen University.

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial-Mesenchymal Transition.
    Article Snippet: .. Dulbecco’sModified Eagle’s Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham’s F12 (Cat. 11320033), Leibovitz’s L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti-β-Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) 2.2. ..

    Recombinant:

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial–Mesenchymal Transition
    Article Snippet: .. Dulbecco's Modified Eagle's Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham's F12 (Cat. 11320033), Leibovitz's L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti- β -Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) .. The clinical tumor and corresponding normal specimens were collected from 98 cases of pancreatic cancer patients, who were operated at the Fuzhou General Hospital (Dongfang Hospital) of Xiamen University.

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial-Mesenchymal Transition.
    Article Snippet: .. Dulbecco’sModified Eagle’s Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham’s F12 (Cat. 11320033), Leibovitz’s L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti-β-Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) 2.2. ..

    Immunohistochemistry:

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial–Mesenchymal Transition
    Article Snippet: .. Dulbecco's Modified Eagle's Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham's F12 (Cat. 11320033), Leibovitz's L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti- β -Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) .. The clinical tumor and corresponding normal specimens were collected from 98 cases of pancreatic cancer patients, who were operated at the Fuzhou General Hospital (Dongfang Hospital) of Xiamen University.

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial-Mesenchymal Transition.
    Article Snippet: .. Dulbecco’sModified Eagle’s Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham’s F12 (Cat. 11320033), Leibovitz’s L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti-β-Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) 2.2. ..

    Luciferase:

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial–Mesenchymal Transition
    Article Snippet: .. Dulbecco's Modified Eagle's Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham's F12 (Cat. 11320033), Leibovitz's L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti- β -Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) .. The clinical tumor and corresponding normal specimens were collected from 98 cases of pancreatic cancer patients, who were operated at the Fuzhou General Hospital (Dongfang Hospital) of Xiamen University.

    Article Title: STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial-Mesenchymal Transition.
    Article Snippet: .. Dulbecco’sModified Eagle’s Medium (DMEM) (Cat. 11965084), RMPI1640 (Cat. 11875093), Ham’s F12 (Cat. 11320033), Leibovitz’s L-15 medium (Cat. 41300070), fetal bovine serum (FBS) (Cat. 10500064), insulin (Cat. A11382IJ), and penicillin-streptomycin (Cat. 15140148) were purchased from Gibco (MA, USA); RNA extraction reagent (Cat. 10606ES60), first strand cDNA synthesis mixture (Cat. 11137ES70), qPCR SYBR Green kit (Cat. 11203ES03), lentivirus concentration solution kit (Cat. 41101ES50), enhanced ECL chemiluminescent substrate kit (Cat. 36222ES76), transferrin (Cat.40102ES60), and cell cycle analysis kit (Cat. 40301ES50) were obtained from Yeasen (Shanghai, China); anti-GAPDH (5174S), anti-Snail (3879S), anti-Vimentin (5741S), anti-E-cadherin (3195S), anti-twist1 (46702S), and anti-β-Catenin (8480S) were purchased from Cell Signaling Technology (MA, USA); recombinant human EGF protein (P01133) was purchased from R&D System; HRP-conjugated goat anti-mouse IgG (Cat. SA00001-1), HRP-conjugated goat anti-rabbit IgG (Cat. SA00001-2), and anti-STC2 antibody (Cat. 60063-1-Ig) were from ProteinTech (Chicago, USA); hydrocortisone (Cat. S1696) was purchased from Selleck (Houston, USA); IHC kit (Cat. KIT-5020) was obtained from MXB Biotechnology (Fuzhou, China); PrimeSTAR HS DNA Polymerase (Cat. R045B) was from Takara Bio (Shiga, Japan); dual luciferase reporter kit (Cat. RG028) was obtained from Beyotime Bio (Shanghai, China); Matrigel matrix (Cat. 356234) was from BD Biosciences (San Jose, CA, USA) 2.2. ..

    Blocking Assay:

    Article Title: STC2 Serves as a Critical Hypoxic Effector in Keloid Pathogenesis by Orchestrating Fibroblasts Activation and ECM Remodelling.
    Article Snippet: Abnormal activation of keloid fibroblasts (KFs) within a hypoxic microenvironment is a hallmark of keloid pathogenesis.. However, the precise molecular mechanisms by which hypoxia drives fibroblast dysfunction remain insufficiently understood.. This study aimed to investigate the role of Stanniocalcin 2 (STC2), a hypoxiaresponsive glycoprotein, in modulating keloid fibroblast behaviour under hypoxic conditions and to elucidate its upstream and downstream regulatory networks.

    Incubation:

    Article Title: STC2 Serves as a Critical Hypoxic Effector in Keloid Pathogenesis by Orchestrating Fibroblasts Activation and ECM Remodelling.
    Article Snippet: Abnormal activation of keloid fibroblasts (KFs) within a hypoxic microenvironment is a hallmark of keloid pathogenesis.. However, the precise molecular mechanisms by which hypoxia drives fibroblast dysfunction remain insufficiently understood.. This study aimed to investigate the role of Stanniocalcin 2 (STC2), a hypoxiaresponsive glycoprotein, in modulating keloid fibroblast behaviour under hypoxic conditions and to elucidate its upstream and downstream regulatory networks.

    Staining:

    Article Title: STC2 Serves as a Critical Hypoxic Effector in Keloid Pathogenesis by Orchestrating Fibroblasts Activation and ECM Remodelling.
    Article Snippet: Abnormal activation of keloid fibroblasts (KFs) within a hypoxic microenvironment is a hallmark of keloid pathogenesis.. However, the precise molecular mechanisms by which hypoxia drives fibroblast dysfunction remain insufficiently understood.. This study aimed to investigate the role of Stanniocalcin 2 (STC2), a hypoxiaresponsive glycoprotein, in modulating keloid fibroblast behaviour under hypoxic conditions and to elucidate its upstream and downstream regulatory networks.

    Control:

    Article Title: STC2 Serves as a Critical Hypoxic Effector in Keloid Pathogenesis by Orchestrating Fibroblasts Activation and ECM Remodelling.
    Article Snippet: Abnormal activation of keloid fibroblasts (KFs) within a hypoxic microenvironment is a hallmark of keloid pathogenesis.. However, the precise molecular mechanisms by which hypoxia drives fibroblast dysfunction remain insufficiently understood.. This study aimed to investigate the role of Stanniocalcin 2 (STC2), a hypoxiaresponsive glycoprotein, in modulating keloid fibroblast behaviour under hypoxic conditions and to elucidate its upstream and downstream regulatory networks.



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    Image Search Results


    A The CCK-8 assay was conducted to investigate the effect of glucose restriction on the proliferation of SW480/Vector, SW480/FUT3 oe ; LOVO/Vector, LOVO/FUT3 oe ; SW480/NC, SW480/siFUT3; HCT116/NC, HCT116/siFUT3 groups. B The colony-forming assay was performed to assess the impact of glucose restriction on the proliferation of SW480/Vector, SW480/FUT3 oe ; LOVO/Vector, LOVO/FUT3 oe ; SW480/NC, SW480/siFUT3; HCT116/NC, HCT116/siFUT3 groups. C The multicellular spheroid assay was carried out to investigate the effect of glucose restriction on the proliferation of SW480/Vector, SW480/FUT3 oe ; LOVO/Vector, LOVO/FUT3 oe ; SW480/NC, SW480/siFUT3; HCT116/NC, HCT116/siFUT3 groups. D Flow cytometry was utilized to analyze the effect of glucose restriction on the cell cycle of SW480/Vector, SW480/FUT3 oe ; LOVO/Vector, LOVO/FUT3 oe ; SW480/NC, SW480/siFUT3; HCT116/NC, HCT116/siFUT3 groups. E The nude mice xenograft model was used to evaluate the effect of FUT3 on the proliferation of CRC cells in vivo. F Bar chart of the subcutaneous tumor volume. G Immunohistochemistry staining was performed to visualize the expression of FUT3 and Ki67 in tumors. H A bar chart was used to indicate the expression levels of FUT3 and Ki67 in tumors. I Western blot analysis was used to determine the expression levels of FUT3, ATF4, and STC2 in subcutaneous tumors.

    Journal: NPJ Precision Oncology

    Article Title: FUT3 mediated GRP78 fucosylation promotes colorectal cancer survival and proliferation under glucose restriction via PERK/ATF4/STC2 axis

    doi: 10.1038/s41698-025-01216-w

    Figure Lengend Snippet: A The CCK-8 assay was conducted to investigate the effect of glucose restriction on the proliferation of SW480/Vector, SW480/FUT3 oe ; LOVO/Vector, LOVO/FUT3 oe ; SW480/NC, SW480/siFUT3; HCT116/NC, HCT116/siFUT3 groups. B The colony-forming assay was performed to assess the impact of glucose restriction on the proliferation of SW480/Vector, SW480/FUT3 oe ; LOVO/Vector, LOVO/FUT3 oe ; SW480/NC, SW480/siFUT3; HCT116/NC, HCT116/siFUT3 groups. C The multicellular spheroid assay was carried out to investigate the effect of glucose restriction on the proliferation of SW480/Vector, SW480/FUT3 oe ; LOVO/Vector, LOVO/FUT3 oe ; SW480/NC, SW480/siFUT3; HCT116/NC, HCT116/siFUT3 groups. D Flow cytometry was utilized to analyze the effect of glucose restriction on the cell cycle of SW480/Vector, SW480/FUT3 oe ; LOVO/Vector, LOVO/FUT3 oe ; SW480/NC, SW480/siFUT3; HCT116/NC, HCT116/siFUT3 groups. E The nude mice xenograft model was used to evaluate the effect of FUT3 on the proliferation of CRC cells in vivo. F Bar chart of the subcutaneous tumor volume. G Immunohistochemistry staining was performed to visualize the expression of FUT3 and Ki67 in tumors. H A bar chart was used to indicate the expression levels of FUT3 and Ki67 in tumors. I Western blot analysis was used to determine the expression levels of FUT3, ATF4, and STC2 in subcutaneous tumors.

    Article Snippet: The tissue sections were incubated with antibodies against FUT3 (1:500; 67344-1-Ig, proteintech); ATF4 (1:200; 10835-1-AP, proteintech); STC2 (1:200; 60063-1-Ig, proteintech) and Ki-67 (1:4000; 27309-1-AP; proteintech), followed by probing with a secondary antibody and mounting with Diaminobenzidine (DAB).

    Techniques: CCK-8 Assay, Plasmid Preparation, Flow Cytometry, In Vivo, Immunohistochemistry, Staining, Expressing, Western Blot

    A Design scheme of GRP78 exon truncation mutations. B Validation of GRP78 exon truncation mutation efficiency. C Co-immunoprecipitation (coIP) assay demonstrates that FUT3 primarily binds to exon 8 of the GRP78 molecule. D The optimal parameter (lambda) for LASSO regression was selected based on ER stress-related genes. E LASSO coefficient profiles for ER stress-related genes in CRC were obtained. F AUC curves were used to evaluate the prognostic signature of ER stress-related genes. G A forest plot of multivariate Cox regression was used to analyze age, gender, AJCC stage, TNM stage, and risk score. H The expression of nine hub genes was compared between the low and high-risk groups. I The mRNA expression levels of FUT3 and STC2 were correlated in CRC tissues ( n = 38). J Immunohistochemistry staining was performed to explore the correlation of protein expression of FUT3 and STC2 in CRC tissues.

    Journal: NPJ Precision Oncology

    Article Title: FUT3 mediated GRP78 fucosylation promotes colorectal cancer survival and proliferation under glucose restriction via PERK/ATF4/STC2 axis

    doi: 10.1038/s41698-025-01216-w

    Figure Lengend Snippet: A Design scheme of GRP78 exon truncation mutations. B Validation of GRP78 exon truncation mutation efficiency. C Co-immunoprecipitation (coIP) assay demonstrates that FUT3 primarily binds to exon 8 of the GRP78 molecule. D The optimal parameter (lambda) for LASSO regression was selected based on ER stress-related genes. E LASSO coefficient profiles for ER stress-related genes in CRC were obtained. F AUC curves were used to evaluate the prognostic signature of ER stress-related genes. G A forest plot of multivariate Cox regression was used to analyze age, gender, AJCC stage, TNM stage, and risk score. H The expression of nine hub genes was compared between the low and high-risk groups. I The mRNA expression levels of FUT3 and STC2 were correlated in CRC tissues ( n = 38). J Immunohistochemistry staining was performed to explore the correlation of protein expression of FUT3 and STC2 in CRC tissues.

    Article Snippet: The tissue sections were incubated with antibodies against FUT3 (1:500; 67344-1-Ig, proteintech); ATF4 (1:200; 10835-1-AP, proteintech); STC2 (1:200; 60063-1-Ig, proteintech) and Ki-67 (1:4000; 27309-1-AP; proteintech), followed by probing with a secondary antibody and mounting with Diaminobenzidine (DAB).

    Techniques: Biomarker Discovery, Mutagenesis, Immunoprecipitation, Co-Immunoprecipitation Assay, Expressing, Immunohistochemistry, Staining

    A The correlation between the ATF4 and STC2 was explored via the GEPIA. B CO-IP was used to detect the binding of GRP78 and PERK with SW480/Vector and SW480/FUT3 oe groups. C The qRT-PCR results of ATF4 and STC2 were obtained from SW480/Vector, SW480/FUT3 oe , HCT116/NC, and HCT116/siFUT3 groups under high (25 mM) or restricted (2.5 mM) glucose conditions. D Western blot results were obtained for FUT3, GRP78, PERK, p-PERK, ATF4, and STC2 from SW480/Vector, SW480/FUT3 oe , HCT116/NC, and HCT116/siFUT3 groups under high (25 mM) or restricted (2.5 mM) glucose conditions. E CoIP and AAL lectin assays confirm that SGN-2FF specifically inhibits GRP78 fucosylation and reduces PERK dissociation from GRP78. F Western blot analysis of the effect of SGN-2FF-mediated inhibition of GRP78 fucosylation on downstream signaling pathway activation. G Cell cycle assay examining the rescue effect of SGN-2FF on CRC proliferation. H Colony formation assay evaluating the rescue effect of SGN-2FF on CRC proliferation. I CCK8 assay investigating the rescue effect of SGN-2FF on colorectal cancer (CRC) proliferation. J Flow cytometry apoptosis staining assessing the rescue effect of SGN-2FF on CRC apoptosis.

    Journal: NPJ Precision Oncology

    Article Title: FUT3 mediated GRP78 fucosylation promotes colorectal cancer survival and proliferation under glucose restriction via PERK/ATF4/STC2 axis

    doi: 10.1038/s41698-025-01216-w

    Figure Lengend Snippet: A The correlation between the ATF4 and STC2 was explored via the GEPIA. B CO-IP was used to detect the binding of GRP78 and PERK with SW480/Vector and SW480/FUT3 oe groups. C The qRT-PCR results of ATF4 and STC2 were obtained from SW480/Vector, SW480/FUT3 oe , HCT116/NC, and HCT116/siFUT3 groups under high (25 mM) or restricted (2.5 mM) glucose conditions. D Western blot results were obtained for FUT3, GRP78, PERK, p-PERK, ATF4, and STC2 from SW480/Vector, SW480/FUT3 oe , HCT116/NC, and HCT116/siFUT3 groups under high (25 mM) or restricted (2.5 mM) glucose conditions. E CoIP and AAL lectin assays confirm that SGN-2FF specifically inhibits GRP78 fucosylation and reduces PERK dissociation from GRP78. F Western blot analysis of the effect of SGN-2FF-mediated inhibition of GRP78 fucosylation on downstream signaling pathway activation. G Cell cycle assay examining the rescue effect of SGN-2FF on CRC proliferation. H Colony formation assay evaluating the rescue effect of SGN-2FF on CRC proliferation. I CCK8 assay investigating the rescue effect of SGN-2FF on colorectal cancer (CRC) proliferation. J Flow cytometry apoptosis staining assessing the rescue effect of SGN-2FF on CRC apoptosis.

    Article Snippet: The tissue sections were incubated with antibodies against FUT3 (1:500; 67344-1-Ig, proteintech); ATF4 (1:200; 10835-1-AP, proteintech); STC2 (1:200; 60063-1-Ig, proteintech) and Ki-67 (1:4000; 27309-1-AP; proteintech), followed by probing with a secondary antibody and mounting with Diaminobenzidine (DAB).

    Techniques: Co-Immunoprecipitation Assay, Binding Assay, Plasmid Preparation, Quantitative RT-PCR, Western Blot, Inhibition, Activation Assay, Cell Cycle Assay, Colony Assay, CCK-8 Assay, Flow Cytometry, Staining

    A The expression of FUT3 and ATF4 was analyzed in SW480 and HCT116 cells with FUT3 overexpression and knockdown under restricted glucose conditions using nuclear and cytoplasmic separation assays. B The ATF4-binding motif on STC2 was identified using JASPAR. C and D ATF4 binding to predicted sites on STC2 was confirmed by ChIP-qPCR and DNA agarose gel electrophoresis using anti-ATF4, control IgG antibodies, and Histone H3 antibodies. E The luciferase reporter assay was used to determine that the ATF4 binding site (+35 bp) was critical in modulating STC2 transcriptional activity. F An immunofluorescence assay was used to detect the expression and nuclear import of ATF4 under 2.5 mM glucose conditions (WT: Control plasmid encoding firefly luciferase, MUT: Plasmid of interest containing the STC2 promoter upstream of firefly luciferase, Vector: Empty vector control plasmid, ATF4oe: ATF4 overexpression plasmid).

    Journal: NPJ Precision Oncology

    Article Title: FUT3 mediated GRP78 fucosylation promotes colorectal cancer survival and proliferation under glucose restriction via PERK/ATF4/STC2 axis

    doi: 10.1038/s41698-025-01216-w

    Figure Lengend Snippet: A The expression of FUT3 and ATF4 was analyzed in SW480 and HCT116 cells with FUT3 overexpression and knockdown under restricted glucose conditions using nuclear and cytoplasmic separation assays. B The ATF4-binding motif on STC2 was identified using JASPAR. C and D ATF4 binding to predicted sites on STC2 was confirmed by ChIP-qPCR and DNA agarose gel electrophoresis using anti-ATF4, control IgG antibodies, and Histone H3 antibodies. E The luciferase reporter assay was used to determine that the ATF4 binding site (+35 bp) was critical in modulating STC2 transcriptional activity. F An immunofluorescence assay was used to detect the expression and nuclear import of ATF4 under 2.5 mM glucose conditions (WT: Control plasmid encoding firefly luciferase, MUT: Plasmid of interest containing the STC2 promoter upstream of firefly luciferase, Vector: Empty vector control plasmid, ATF4oe: ATF4 overexpression plasmid).

    Article Snippet: The tissue sections were incubated with antibodies against FUT3 (1:500; 67344-1-Ig, proteintech); ATF4 (1:200; 10835-1-AP, proteintech); STC2 (1:200; 60063-1-Ig, proteintech) and Ki-67 (1:4000; 27309-1-AP; proteintech), followed by probing with a secondary antibody and mounting with Diaminobenzidine (DAB).

    Techniques: Expressing, Over Expression, Knockdown, Binding Assay, ChIP-qPCR, Agarose Gel Electrophoresis, Control, Luciferase, Reporter Assay, Activity Assay, Immunofluorescence, Plasmid Preparation

    A qPCR analysis of ATF4 and STC2 mRNA expression levels following PERK inhibitor treatment. B Western blot assessment of PERK/ATF4/STC2 pathway activation upon PERK inhibitor administration. C CCK-8 assay evaluating the rescue effect of PERK inhibition on CRC proliferative capacity. D Colony formation assay demonstrating the restorative effect of PERK inhibition on CRC proliferation. E 3D spheroid formation assay confirming the rescue of CRC proliferative capacity with PERK inhibitor treatment. F Flow cytometric apoptosis analysis showing the rescue of anti-apoptotic capacity by PERK inhibition. G qPCR validation of STC2 knockdown efficiency following siRNA transfection. H Western blot verification of STC2 knockdown efficiency. I CCK-8 assay examining the rescue effect of STC2 knockdown on CRC proliferation. J Colony formation assay demonstrating the restorative effect of STC2 interference on CRC proliferative capacity. K 3D spheroid formation assay confirming the rescue of CRC proliferation following STC2 knockdown. L Flow cytometric apoptosis analysis showing the restoration of anti-apoptotic capacity with STC2 interference.

    Journal: NPJ Precision Oncology

    Article Title: FUT3 mediated GRP78 fucosylation promotes colorectal cancer survival and proliferation under glucose restriction via PERK/ATF4/STC2 axis

    doi: 10.1038/s41698-025-01216-w

    Figure Lengend Snippet: A qPCR analysis of ATF4 and STC2 mRNA expression levels following PERK inhibitor treatment. B Western blot assessment of PERK/ATF4/STC2 pathway activation upon PERK inhibitor administration. C CCK-8 assay evaluating the rescue effect of PERK inhibition on CRC proliferative capacity. D Colony formation assay demonstrating the restorative effect of PERK inhibition on CRC proliferation. E 3D spheroid formation assay confirming the rescue of CRC proliferative capacity with PERK inhibitor treatment. F Flow cytometric apoptosis analysis showing the rescue of anti-apoptotic capacity by PERK inhibition. G qPCR validation of STC2 knockdown efficiency following siRNA transfection. H Western blot verification of STC2 knockdown efficiency. I CCK-8 assay examining the rescue effect of STC2 knockdown on CRC proliferation. J Colony formation assay demonstrating the restorative effect of STC2 interference on CRC proliferative capacity. K 3D spheroid formation assay confirming the rescue of CRC proliferation following STC2 knockdown. L Flow cytometric apoptosis analysis showing the restoration of anti-apoptotic capacity with STC2 interference.

    Article Snippet: The tissue sections were incubated with antibodies against FUT3 (1:500; 67344-1-Ig, proteintech); ATF4 (1:200; 10835-1-AP, proteintech); STC2 (1:200; 60063-1-Ig, proteintech) and Ki-67 (1:4000; 27309-1-AP; proteintech), followed by probing with a secondary antibody and mounting with Diaminobenzidine (DAB).

    Techniques: Expressing, Western Blot, Activation Assay, CCK-8 Assay, Inhibition, Colony Assay, Tube Formation Assay, Biomarker Discovery, Knockdown, Transfection